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w w β mercaptoethanol  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology w w β mercaptoethanol
    W W β Mercaptoethanol, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/w+w+%CE%B2+mercaptoethanol/%CE%B2-Mercaptoethanol/pmc04747838-88-18-36
    Average 94 stars, based on 234 article reviews
    w w β mercaptoethanol - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Stripping Membranes:

    Article Title: A cyclin-dependent kinase inhibitor, dinaciclib, impairs homologous recombination and sensitizes multiple myeloma cells to PARP inhibition
    Article Snippet: Protein signals were detected by chemiluminescence using an ECL detection reagent (Bio-Rad). .. After removing antibodies in stripping buffer (2% w/v sodium dodecyl sulfate [SDS]; 62.5-mM Tris-HCl pH 6.8; and 0.7% [w/w] β-mercaptoethanol) for 15 min at 50°C, the membrane was re-probed with primary antibodies to BRCA1 and β-actin (Santa Cruz Biotechnology) and detected as above. .. RAD51 and pBRCA1 protein band densities were quantified using Quantity One software (Bio-Rad) and the values expressed relative to actin and total BRCA1, respectively (controls for loading and transfer).

    Membrane:

    Article Title: A cyclin-dependent kinase inhibitor, dinaciclib, impairs homologous recombination and sensitizes multiple myeloma cells to PARP inhibition
    Article Snippet: Protein signals were detected by chemiluminescence using an ECL detection reagent (Bio-Rad). .. After removing antibodies in stripping buffer (2% w/v sodium dodecyl sulfate [SDS]; 62.5-mM Tris-HCl pH 6.8; and 0.7% [w/w] β-mercaptoethanol) for 15 min at 50°C, the membrane was re-probed with primary antibodies to BRCA1 and β-actin (Santa Cruz Biotechnology) and detected as above. .. RAD51 and pBRCA1 protein band densities were quantified using Quantity One software (Bio-Rad) and the values expressed relative to actin and total BRCA1, respectively (controls for loading and transfer).

    Article Title: A Small-Molecule Inhibitor of RAD51 Reduces Homologous Recombination and Sensitizes Multiple Myeloma Cells to Doxorubicin
    Article Snippet: Membranes were washed in TBST, incubated with HRP-conjugated goat anti-mouse IgG (Santa Cruz Biotechnology), washed again, and signal detected by chemiluminescence using ECL detection reagent (Bio-Rad). .. Antibodies were removed from the membrane by incubation in a solution containing 2% (w/v) sodium docecylsulfate, 62.5 mM Tris-HCl pH6.8, and 0.7% (w/w) β-mercaptoethanol for 15 min at 50°C and the membrane re-probed with primary antibody to actin (Santa Cruz Biotechnology) and signal detected as described above. .. Band intensities were quantified using Quantity One software (Bio-Rad) and RAD51 intensity in each lane was expressed relative to the corresponding actin band and normalized to vehicle treatment.

    Incubation:

    Article Title: A Small-Molecule Inhibitor of RAD51 Reduces Homologous Recombination and Sensitizes Multiple Myeloma Cells to Doxorubicin
    Article Snippet: Membranes were washed in TBST, incubated with HRP-conjugated goat anti-mouse IgG (Santa Cruz Biotechnology), washed again, and signal detected by chemiluminescence using ECL detection reagent (Bio-Rad). .. Antibodies were removed from the membrane by incubation in a solution containing 2% (w/v) sodium docecylsulfate, 62.5 mM Tris-HCl pH6.8, and 0.7% (w/w) β-mercaptoethanol for 15 min at 50°C and the membrane re-probed with primary antibody to actin (Santa Cruz Biotechnology) and signal detected as described above. .. Band intensities were quantified using Quantity One software (Bio-Rad) and RAD51 intensity in each lane was expressed relative to the corresponding actin band and normalized to vehicle treatment.



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